As probably none of you know, I inherited Jen’s thesis dealing with oil biodegradation by fungi. With the expert help of post-doc Rachel, we grow three different species of fungus on agar and then place a small plug of this on a filter over oiled sand:
This semester, I was investigating temperature as an environmental factor for fungal growth via incubations of fungi and oiled sand that were left to grow for 6 weeks. The experiments covered 4 temperatures, three species of fungus, triplicates of each species at each temperature, oiled sand in artificial seawater (ASW), C17/C18/pristane/phytane (compounds) in ASW, oiled sand in fresh water, and the controls. When all was said and done, I had 132 individual samples incubating simultaneously. And I had to liquid extract and run all of them on the GC, then analyze everything. This is why I am grateful to the minions.
Not THOSE minions, but Chloe, Sherlande, Liana, and Katie:
These past two weeks have been incredibly intense in terms of lab work and I am eternally grateful to these wonderful people for helping me out! Many thanks, minions! Good luck on your finals!